The cuticle, a complex network of polymers, covered and impregnated by waxes, covers the aerial organs of all plants. The cuticle serves essential purposes for the plant, such as reducing water loss and providing protection against pathogens or other environmental stresses [1]. The main component of the cuticle is cutin, a polymer that consists primarily of esters of mono-, di- and tri- hydroxy fatty acids and glycerol. Cutin presents unique properties, and can be used as a basis to produce new durable and flexible, yet biodegradable polymeric materials [2]. A key enzyme in cutin biosynthesis, CUS1, was identified in a collaborative research carried between DTU Chemistry and the Institute of Biotechnology at Cornell University [3]. CUS1 was successfully used to synthesize in vitro oligomers of 2-mono(10,16-dihydroxyhexadecanoyl)glycerol (2-MHG), the most abundant monomer of cutin in most plants. Isotopically labelled 2-MHG could be the key to decipher some of the main incognita regarding cutin synthesis. On the one hand, deuterium labelled 2-MHG could answer questions regarding the selectivity of CUS1 through in vitro mass spectroscopy studies of the formed oligomers. On the other hand, tritium labelled 2-MHG will help understand the monomer transport process in planta. We have developed an easy route to both isotopically labeled molecules.